Method measuring cellulose developed

The CADoS process is characterised by the separation of cellulose and its subsequent use for the dewatering of sewage sludge. To optimise the process it is important to find out more about the quantity of cellulose brought in with the wastewater during the day.

After a stock-taking exercise by Groningen University (RUG) it emerged that there was no generally used and reliable method for measuring the cellulose. This resulted in RUG (Lena Faust) embarking on the development of a method, in which she succeeded. There is now a reliable method for measuring cellulose in wastewater and sludge. The method uses the enzymatic conversion of cellulose to glucose, which is easily measurable. Pre-treatment of the sample and use of the right enzyme were also part of the development process. More details about the method can be found in the poster presented at the Cellulose Symposium on 19 June 2014: Poster_Cellulose symposium

Meetmethode concentratie cellulose - RUG

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One Comment

  1. Hello,
    I am a PhD student at Western University, Canada. Just today, I came across your poster on “A new and reliable method to measure cellulose in wastewater and sludge”. I am very much interested in knowing the procedure in detail to duplicate your methodology in our lab here.
    In step 3 of the “Methods” section of your poster:
    3. Digestion of known amount (e.g. 20 mg) sieved material with cellulase from T. reseei (Sigma Aldrich ) at 40 ˚C in water/buffer in stirred tubes
    What is the ratio of sieved material and cellulase do you add?
    What is the volume of water or buffer used?
    Does the pH of the digestion mixture affect?
    Is 170 h the optimal time to run the digestion for?
    I would greatly appreciate your guidance.
    Medhavi Gupta
    PhD Candidate
    Environmental Engineering
    Western University
    London, ON

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